LOCUS       40924_49132        4012 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD024, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 4012)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4012)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    46..51
                     /label=ZraI
                     /note="ZraI"
     misc_feature    49..69
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a
                     BHR replicons, neutral sites, or cyanobacterial replicons; 
                     synthetic"
     misc_feature    70..75
                     /label=XbaI
                     /note="XbaI"
     misc_recomb     76..877
                     /note="S7942NS3-RA; right arm of the homologous
                     recombination site NS3 into the chromosome of Synechococcus
                     elongatus PCC7942; from pHN1_lacUV5, INSD accession 
                     NC_007604, HM026758"
     rep_origin      914..1502
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(1688..1828)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     misc_recomb     1910..2801
                     /note="S7942NS3-LA; left arm of the homologous
                     recombination site NS3 into the chromosome of Synechococcus
                     elongatus PCC7942; from pHN1_lacUV5, INSD accession 
                     NC_007604, HM026758"
     misc_feature    2802..2807
                     /label=NheI
                     /note="NheI"
     misc_feature    2808..2828
                     /note="GC; GC-adaptor for seamless assembly, flanking BHR 
                     replicons or neutral sites; synthetic"
     misc_feature    2826..2831
                     /label=ZraI
                     /note="ZraI"
     CDS             complement(2955..3812)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3813..3917)
                     /label=AmpR promoter
     misc_feature    3943..3948
                     /label=ZraI
                     /note="ZraI"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagcgacgt cgggggcccc
       61 cggggggatt ctagacgtga gccgtagtcc cgtcgccaga cgcgggtgcc cacgggcgtc
      121 gtcaggatgt ccgtaattga ctgccggagg tggtcaatgc ccttcagctc cttgccactg
      181 tcacggctca tgcctcgggt cattagtcgc ccgctccggt atcttcactg gcttcgatga
      241 ttgccgcccc gcagctgcag aggtcaccga tccgagcagt cggcctctgg ttggtaaaga
      301 ccgtgcgact gccggtgatg atcgtgttca agccatgcag ggggcaggcg tgaaggtcat
      361 cctttcgggc cacggggcgg ctgttgacga aggtgtcgtc gctcccggtg atgatgatcc
      421 cgccgtgatc ggtcacgtcg tttagtcgag cgatgcctgg cgtcgtagtc acgggtttag
      481 gtcaatacga cttgcggtca ctgtaacgtt gccctcggcg gtcacgttaa cgtcgccttg
      541 ggcttcgact tgcgcctcct gcacaaggat cacaatccgt ccttgggctg cggtgaggtc
      601 gatcttgtac tcatgcgctt cgcggtcgta ctggatgatt gagtcatcct cgaactgcgt
      661 cttttggatc gtttctttgt cctcgatctg ggggtagtca gtcgagaacg cgccgggcat
      721 cgcgaagccc tgactgatct cgccggaggg ggccatcacg acgacggcct caccgacctc
      781 gggcgcccac cagaaccgat ccttgcccgc tcgctgcgtg agccacggaa tccagtcagt
      841 gaggagcagc gcctcgccgc tctcctcgtc ttcctcgagc gtcagacccc gtagaaaaga
      901 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      961 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1021 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     1081 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1141 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1201 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1261 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1321 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1381 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1441 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     1501 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1561 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1621 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1681 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     1741 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     1801 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     1861 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctccgcggc gtcgtgatgt
     1921 cgaaacccaa gccaccctca gaggtgaagg ccgcttcgag cacatcggga agcgtgtcga
     1981 cgacggtgct cggtgcctcg ggtaagagcc agatagatgc ggtgacgttc accgtcgcga
     2041 tcgtggcggg aaccacggtc actgcgtccg ttacaactcg cacgtcgtcg gccagcacga
     2101 ctgactcgac ggcttcgatt agcccgggag aggcaaggcc atcaggctcg gtagacagaa
     2161 tgctgattaa cacctcgccg ggagcagggc tgctcacagc cgcgtccttc acccgtgggt
     2221 cagccgtcag cgcttgatag cggtaccagg ccgcaccgcc tgcggtcgag ctgcccttga
     2281 tccgctcgat ggtgcgatcg cgaagctcgc catccggctc attcggctgc cgcaccacgc
     2341 cgtagaacgc ggcaaggttg tcgaggtcgg ggccgtttga gtagcgaagc agtgtcgcaa
     2401 gaagtgcgtc gttgatccgc tgacgcagga tcagctcgcg ggctgcgcag acctccagca
     2461 gcttgatgac cgggtcggat tcgaggatgg cggtgtagct ggcgtcacgc gatcgcaggt
     2521 cgtcgatcaa gtcctgcagg atcagttcaa agtccagcgc ttcgatgatg gtgggcgcgg
     2581 gaatcgtagc aaagtcaaga acggtcatga gacgactaag ccctccagcg tgatacgcct
     2641 gccctcgggg atgtagtagc cgatcaggtt cagctcgact tgaccagctg cgctggctga
     2701 gacgatgcga accttctcca gcttcagccg tggctcccag cgatcgagcg cttcagctgt
     2761 ggccgccacc aggtcaacga tgagggactg gttgatcggt cgctagcatc gcgcgcgcgc
     2821 gcgcggacgt cctcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     2881 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     2941 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3001 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3061 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3121 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3181 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3241 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3301 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3361 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3421 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3481 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     3541 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     3601 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     3661 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     3721 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     3781 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     3841 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     3901 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     3961 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
